Runwen Yao
Biomolecular condensates · mesoscale organization · cellular function
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Scientific Imaging

Microscopy, condensates, and cellular organization viewed through the experimental systems that shape my research.

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3D-SIM

Vibrio parahaemolyticus

PKMO FX (magenta), protein of interest (green), and DAPI (blue)

light-sheet microscopy

72 hpf zebrafish

RNA (magenta), Nucleoli (green), Pol II (red), Nuclei (blue)

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TIRF-SIM

Actin and Nck

LifeAct-labeled actin (magenta)， Nck (green)

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3D-SIM

Nucleolar organization

FBL (green), RPA194 (red), Nuclei (blue)

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3D-SIM

FC/DFC in the nucleolus

FBL (green), RPA194 (magenta)

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TIRF-SIM

Actin cytoskeleton architecture

Phalloidin-labeled actin (blue)

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STED

Paraspeckle spatial organization

NEAT1_5′/3′ (green), NEAT1_mid (magenta)

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smFISH

MALAT1

MALAT1 (white), DAPI (blue)

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smFISH

NEAT1

NEAT1 (white), DAPI (blue)

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TIRF-SIM

Actin cytoskeleton in U2OS

LifeAct-labeled actin (yellow)

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3D-SIM

DDX21 ring structures

DDX21 (green), FLAG-tagged protein (red)

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SMLM

Nascent pre-rRNA

Color encodes depth